Comparison of protocols for depleting anti- E. coli antibody in immunoblotting of recombinant antigens

A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti- E. coli antibodies from human sera are provided and their us...

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Veröffentlicht in:	Journal of immunological methods 1996-03, Vol.190 (1), p.143-145
Hauptverfasser:	Covini, Giovanni, Chan, Edward K.L., Colombo, Massimo, Tan, E.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Absorption Anti- E. coli antibody depletion Antibodies, Bacterial - immunology Autoantibody Biological and medical sciences Blotting, Western - methods Escherichia coli Escherichia coli - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunoblotting Molecular immunology Recombinant Proteins - immunology Techniques
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Beschreibung
Zusammenfassung:	A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti- E. coli antibodies from human sera are provided and their uses are discussed.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(95)00288-X