A Role for CD81 in Early T Cell Development

Early stages of T cell development are thought to include a series of coordinated interactions between thymocytes and other cells of the thymus. A monoclonal antibody specific for mouse CD81 was identified that blocked the appearance of αβ but not γδ T cells in fetal organ cultures initiated with da...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1996-01, Vol.271 (5246), p.198-200
Hauptverfasser:	Boismenu, Richard, Rhein, Maria, Fischer, Wolfgang H., Havran, Wendy L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antigens, CD - genetics Antigens, CD - immunology Antigens, CD - physiology Base Sequence Biological and medical sciences CD4 Antigens - analysis CD8 Antigens - analysis Cell Differentiation Cell lines CHO Cells Cricetinae Cultured cells Developmental Stages Differentiation Epithelial cells Fundamental and applied biological sciences. Psychology Fundamental immunology Immunity (Disease) Immunobiology Ligands Lymphatic system Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Medical research Membrane Proteins - immunology Membrane Proteins - physiology Mice Molecular Sequence Data Organ Culture Techniques Pregnancy Receptors, Antigen, T-Cell, alpha-beta - analysis Receptors, Antigen, T-Cell, gamma-delta - analysis Spleen cells Stromal cells Stromal Cells - immunology T cell antigen receptors T cells T lymphocytes T-Lymphocyte Subsets - cytology T-Lymphocyte Subsets - immunology Tetraspanin 28 Thymocytes Thymus Gland - cytology Thymus Gland - immunology Transfection
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Beschreibung
Zusammenfassung:	Early stages of T cell development are thought to include a series of coordinated interactions between thymocytes and other cells of the thymus. A monoclonal antibody specific for mouse CD81 was identified that blocked the appearance of αβ but not γδ T cells in fetal organ cultures initiated with day 14.5 thymus lobes. In reaggregation cultures with CD81-transfected fibroblasts, CD4$^-$ CD8$^-$ thymocytes differentiated into CD4$^+$ CD8$^+$ T cells. Thus, interactions between immature thymocytes and stromal cells expressing CD81 are required and may be sufficient to induce early events associated with T cell development.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.271.5246.198