Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter
We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and...
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Veröffentlicht in: | Gene 1987, Vol.60 (1), p.65-74 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We report the cloning of the mouse
pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the
pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse
pgk-1 gene is homologous to the human
pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the
pgk-1 gene. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(87)90214-9 |