Isolation and characterization of brush border membrane vesicles from pig small intestine

1. 1. Brush border membrane vesicles (BBMV) were isolated from swine mid-intestine by a MgCl 2 precipitation and sucrose density gradient centrifugation. 2. 2. Transport of d-glucose and l-alanine were Na +-stimulated and into an osmotically sensitive space. 3. 3. Estimates of kinetic parameters for...

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Veröffentlicht in:	Comparative biochemistry and physiology. A, Comparative physiology Comparative physiology, 1987, Vol.88 (3), p.511-517
Hauptverfasser:	Moe, Aaron J, Jackson, Michael J
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - metabolism Animals Biological and medical sciences Cell Fractionation Centrifugation, Density Gradient - methods characterization Fundamental and applied biological sciences. Psychology Glucose - metabolism Intestine, Small - ultrastructure Intestine. Mesentery isolation Kinetics Membrane Potentials membranes Microvilli - metabolism Microvilli - physiology Microvilli - ultrastructure small intestine Swine Vertebrates: digestive system vesicles
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Zusammenfassung:	1. 1. Brush border membrane vesicles (BBMV) were isolated from swine mid-intestine by a MgCl 2 precipitation and sucrose density gradient centrifugation. 2. 2. Transport of d-glucose and l-alanine were Na +-stimulated and into an osmotically sensitive space. 3. 3. Estimates of kinetic parameters for Na +-dependent d-glucose transport were: apparent K t = 1.8 mM and J max = 16.8 nmol/mg protein/min. 4. 4. Results of experiments with the ΔpH sensitive fluorescent probe 9-aminoacricline indicated indepen- dent mechanisms for Na +-dependent glucose transport and Na +/H + exchange. 5. 5. This study demonstrates that pig BBMV provide a useful model for investigating intestinal membrane transport.
ISSN:	0300-9629
DOI:	10.1016/0300-9629(87)90073-9