Long-term in vitro cultivation of Plasmodium berghei

Long-term in vitro cultivation of Plasmodium berghei

Long-term in vitro culture of Plasmodium berghei was established using the Petri dish candle jar method of Trager and Jensen (1976). Cultures were established at 22, 27 and 37°C. As optimal growth was observed at 27°C, subsequent cultivation was carried out at this temperature. RPMI 1640 medium was...

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Veröffentlicht in:International journal for parasitology 1987-10, Vol.17 (7), p.1329-1331
Hauptverfasser: Ramaiya, Mira L., Kamath, Vijayalaxmi R., Renapurkar, D.M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Culture Media
Erythrocytes - parasitology
Fundamental and applied biological sciences. Psychology
General aspects and techniques
long-term in vitro culture
Malaria
Mice
Plasmodium berghei
Plasmodium berghei - growth & development
Protozoa
Temperature
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Zusammenfassung:Long-term in vitro culture of Plasmodium berghei was established using the Petri dish candle jar method of Trager and Jensen (1976). Cultures were established at 22, 27 and 37°C. As optimal growth was observed at 27°C, subsequent cultivation was carried out at this temperature. RPMI 1640 medium was modified by incorporating additional glucose (1 mg ml −1) and bactopeptone (1 mg ml −1) in the medium. This medium was found suitable for maintenance of mouse erythrocytes in vitro. P. berghei cultures were maintained using candle jars and this modified RPMI 1640 medium for 45 weeks.
ISSN:0020-7519
1879-0135
DOI:10.1016/0020-7519(87)90099-3