A zebrafish homeobox-containing gene with embryonic transcription

A genomic library of zebrafish ( Brachydanio rerio ) was constructed and screened with homeobox-containing probes. One of the strongly cross-hybridizing clones was characterized by DNA sequencing. The deduced amino acid sequence exhibits extensive homology (>80%) relative to the Antennapedia-clas...

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Veröffentlicht in:	Biochemical and biophysical research communications 1987-12, Vol.149 (3), p.1165-1171
Hauptverfasser:	Eiken, Hans G., Njøstad, Pål R., Molven, Anders, Fjose, Anders
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Base Sequence DNA, Recombinant Drosophila Drosophila melanogaster - genetics Embryo, Nonmammalian - analysis Embryonic and Fetal Development Exact sciences and technology Fishes - embryology Fishes - genetics Genes, Homeobox Marine Molecular Sequence Data Nucleic Acid Hybridization Other techniques and industries Poly A - analysis RNA, Messenger - analysis Sequence Homology, Nucleic Acid
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Zusammenfassung:	A genomic library of zebrafish ( Brachydanio rerio ) was constructed and screened with homeobox-containing probes. One of the strongly cross-hybridizing clones was characterized by DNA sequencing. The deduced amino acid sequence exhibits extensive homology (>80%) relative to the Antennapedia-class of Drosophila homeobox sequences. Characterization of the gene with respect to expression demonstrated that two transcripts of 2.1 and 1.4 kb, respectively, are present in embryonic poly(A +)RNA. The highest concentration of the two RNA species was observed in embryos which have terminated the process of somite formation.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(87)90530-4