Dog alpha-1-acid glycoprotein: Purification and biochemical characterization

Dog alpha-1-acid glycoprotein was purified to homogeneity from dog serum in a three-step procedure involving precipitation with sulphosalicylic acid, isoelectric focusing and size exclusion chromatography. The molecular heterogeneity in the peptide part and in the carbohydrate part of the molecule w...

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Veröffentlicht in:	Journal of pharmacological methods 1987-12, Vol.18 (4), p.335-345
Hauptverfasser:	Dello, Carine P., Belpaire, Frans M., Kint, Jos A., Fraeyman, Norbert H.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha 1-glycoprotein Alpha-1-acid glycoprotein Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Concanavalin A - pharmacology Dog Dogs Fundamental and applied biological sciences. Psychology Glycoproteins Immunoelectrophoresis, Two-Dimensional Indicators and Reagents Isoelectric Focusing Orosomucoid - analysis Orosomucoid - immunology Orosomucoid - isolation & purification Oxprenolol - metabolism Protein Binding Proteins Purification
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Zusammenfassung:	Dog alpha-1-acid glycoprotein was purified to homogeneity from dog serum in a three-step procedure involving precipitation with sulphosalicylic acid, isoelectric focusing and size exclusion chromatography. The molecular heterogeneity in the peptide part and in the carbohydrate part of the molecule was investigated with analytical isoelectric focusing in a narrow pH range and crossed immunoaffinity electrophoresis with concanavalin A (con A) in the first-dimension gel. Up to seven molecular forms with different isoelectric points were found, whereas only a single con A-dependent molecular form was detected.
ISSN:	0160-5402
DOI:	10.1016/0160-5402(87)90065-9