Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA

The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation c...

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Veröffentlicht in:Molecular biotechnology 1995-12, Vol.4 (3), p.315-317
Hauptverfasser: PRITCHARD, K. H, SEIFERT, H. S
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Electrophoresis, Agar Gel
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Neisseria gonorrhoeae - genetics
Plasmids - genetics
Transformation, Bacterial
Ultraviolet Rays
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02779023