Monoclonal antibodies against pig transferrin: blocking and binding activity

Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin b...

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Veröffentlicht in:	Folia microbiologica 1987, Vol.32 (5), p.448-452
Hauptverfasser:	TREBICHAVSKY, I, MACKU, B, VIKLICKY, V, KOUKAL, M, SIMAN, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Newborn Antibodies, immunoglobulins Antibodies, Monoclonal Antigen-Antibody Complex Biological and medical sciences Cell Membrane - metabolism Cells, Cultured Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Fundamental immunology Hybridomas - immunology Lymphocytes - metabolism Molecular immunology Monoclonal antibodies Receptors, Transferrin - metabolism Swine Swine, Miniature Transferrin - immunology Transferrin - metabolism
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Zusammenfassung:	Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin bound to specific receptors on the plasma membrane. The binding of iodinated pig transferrin to PK cells was studied. It could be blocked by non-labelled transferrin in excess, by pig serum or by anti-pig transferrin monoclonal antibodies. PTF-03 expressed the lowest blocking activity among the antibodies tested.
ISSN:	0015-5632 1874-9356
DOI:	10.1007/BF02887578