One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin

The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.

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Bibliographische Detailangaben
Veröffentlicht in:	Molecular biotechnology 1995-12, Vol.4 (3), p.247-258
Hauptverfasser:	CROWE, J, MASONE, B. S, RIBBE, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - biosynthesis Biological and medical sciences Biotechnology Chelating Agents Chromatography, Affinity Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Vectors - chemistry Histidine - chemistry Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Nitrilotriacetic Acid - analogs & derivatives Nitrilotriacetic Acid - chemistry Organometallic Compounds - chemistry Plasmids - genetics Protein Denaturation Recombinant Proteins - isolation & purification Recombination, Genetic
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Beschreibung
Zusammenfassung:	The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.
ISSN:	1073-6085 1559-0305
DOI:	10.1007/BF02779018