Antibiotic-resistance cassettes for Bacillus subtilis

Antibiotic-resistance cassettes for Bacillus subtilis

The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromoso...

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Veröffentlicht in:Gene 1995-12, Vol.167 (1), p.335-336
Hauptverfasser: Guérout-Fleury, Anne-Marie, Shazand, Kamran, Frandsen, Niels, Stragier, Patrick
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus subtilis - genetics
Base Sequence
chromosomal disruption
Drug Resistance, Microbial
Erythromycin
Genetic Vectors
kanamycin
Molecular Sequence Data
Plasmids
spectinomycin
tetracycline
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Beschreibung
Zusammenfassung:The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00652-4