Crystallization and Preliminary X-Ray Studies of Isocitrate Dehydrogenase from Thermus thermophilus HB8

Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photograp...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biochemistry (Tokyo) 1995-10, Vol.118 (4), p.679-680
Hauptverfasser: Ohzeki, Masato, Yaoi, Takuro, Moriyama, Hideaki, Oshima, Tairo, Tanaka, Nobuo
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I212121 space group with unit cell dimensions of a =100.1 Å, b=150.4 A, and c=87.4 Å. Intensity data were collected up to 2.5 Å resolution.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a124963