Crystallization and Preliminary X-Ray Studies of Isocitrate Dehydrogenase from Thermus thermophilus HB8
Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photograp...
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Veröffentlicht in: | Journal of biochemistry (Tokyo) 1995-10, Vol.118 (4), p.679-680 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6×0.6×0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I212121 space group with unit cell dimensions of a =100.1 Å, b=150.4 A, and c=87.4 Å. Intensity data were collected up to 2.5 Å resolution. |
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ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/oxfordjournals.jbchem.a124963 |