Localization of lipase genes on Candida rugosa chromosomes

Localization of lipase genes on Candida rugosa chromosomes

In the yeast Candida rugosa the lipase isozymes are encoded by a family of genes, five of which have been cloned and sequenced in our laboratory. This paper reports on the identification and preliminary characterization of two new related sequences, thus extending this multigene family to seven memb...

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Veröffentlicht in:Current genetics 1995-10, Vol.28 (5), p.454-457
Hauptverfasser: Brocca, S, Grandori, R, Breviario, D, Lotti, M
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Candida - enzymology
Candida - genetics
Candida rugosa
chromosome
Chromosome Mapping
chromosomes
Chromosomes, Fungal
cromosomas
DNA, Recombinant
gene
gene location
genes
Genome, Fungal
Lipase - genetics
localisation de gene
localizacion de genes
Molecular Sequence Data
triacilglicerol lipasa
triacylglycerol lipase
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Zusammenfassung:In the yeast Candida rugosa the lipase isozymes are encoded by a family of genes, five of which have been cloned and sequenced in our laboratory. This paper reports on the identification and preliminary characterization of two new related sequences, thus extending this multigene family to seven members. The total DNA content of Candida cells was estimated by laser flow-cytometry at about 20 Mb. Eight chromosomes with sizes ranging between 100 kb and 2.1 Mb, as determined by comparison with S. cerevisiae chromosomal bands, were resolved by pulsed-field gel electrophoresis. The lipase-encoding genes were localized on chromosome 1, therefore suggesting that they have originated through multiple duplication events of an ancestral gene.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00310815