Specific staining of glycine N-methyltransferase

Glycine N‐methyltransferase from rabbit, human, rat and pig livers was separated by isoelectric focusing and a specific functional staining method was developed through the detection of sarcosine produced from the methylation of glycine. Isozyme patterns obtained in the various species tested differ...

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Veröffentlicht in:	Electrophoresis 1995, Vol.16 (1), p.1898-1899
Hauptverfasser:	Santos, Fátima, Amorim, António, Kömpf, Jost
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Glycine - metabolism Glycine N-Methyltransferase Humans Isoelectric Focusing - methods Isoenzymes - isolation & purification Isoenzymes - metabolism Liver Liver - enzymology Methylation Methyltransferases - isolation & purification Methyltransferases - metabolism Rabbits Rats S-Adenosylmethionine Sarcosine - analysis Sarcosine - metabolism Staining and Labeling Swine Transmethylation Zymogram
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Beschreibung
Zusammenfassung:	Glycine N‐methyltransferase from rabbit, human, rat and pig livers was separated by isoelectric focusing and a specific functional staining method was developed through the detection of sarcosine produced from the methylation of glycine. Isozyme patterns obtained in the various species tested differ both in the number of bands and apparent isoelectric points. These differences may explain the contradictory data on the subunit structure and glycosylation status of the enzyme reported so far.
ISSN:	0173-0835 1522-2683
DOI:	10.1002/elps.11501601312