A simple method for the quantitative evaluation of functional effects of xenobiotics with cultured cells

We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or...

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Veröffentlicht in:	Cell biology and toxicology 1994-12, Vol.10 (5-6), p.305-309
Hauptverfasser:	LEES, D, PUGNERE, D, CASTEL, A, RAUX, O, TRAVO, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cells, Cultured - cytology Cells, Cultured - drug effects Cells, Cultured - metabolism General aspects. Methods Image Processing, Computer-Assisted - instrumentation Medical sciences Microscopy, Video - instrumentation Microscopy, Video - methods Muscle Contraction - drug effects Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Rats Toxicology Xenobiotics - pharmacology
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Beschreibung
Zusammenfassung:	We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time intervals ranging from 10 s to 6 h or more, over periods of time ranging from a few tens of minutes to 3-4 days. Any aspect of cell physiological behavior can be studied (differentiation-dedifferentiation, migration, division, degeneration, death) without preliminary staining and/or fixation provided it results in optically visible changes.
ISSN:	0742-2091 1573-6822
DOI:	10.1007/BF00755775