Onset of Pro-Thyrotropin-Releasing Hormone Gene Expression in Cultured Rat Anterior Pituitary Cells Is Expedited by Dexamethasone

Onset of Pro-Thyrotropin-Releasing Hormone Gene Expression in Cultured Rat Anterior Pituitary Cells Is Expedited by Dexamethasone

The early onset of proTRH gene expression in anterior pituitary (AP) cells in culture and its regulation by dexamethasone (DEX) were investigated. AP cells derived from 15-day-old rats were cultured for up to 4 days in the presence or absence of 10-7M DEX. TRH peptide levels, which could be detected...

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Veröffentlicht in:Molecular and cellular neuroscience 1994-12, Vol.5 (6), p.678-683
Hauptverfasser: Rondeel, Jan M.M., Bruhn, Thomas O., Bolduc, Thomas G., Jackson, Ivor M.D.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cells, Cultured
Dexamethasone - pharmacology
Gene Expression - drug effects
In Situ Hybridization
Male
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - physiology
Protein Precursors - genetics
Pyrrolidonecarboxylic Acid - analogs & derivatives
Rats
Thyrotropin-Releasing Hormone - genetics
Time Factors
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Zusammenfassung:The early onset of proTRH gene expression in anterior pituitary (AP) cells in culture and its regulation by dexamethasone (DEX) were investigated. AP cells derived from 15-day-old rats were cultured for up to 4 days in the presence or absence of 10-7M DEX. TRH peptide levels, which could be detected only after 3 days of culture in control cells, were detectable after 1 day in DEX-treated cells. Levels rose from undetectable (
ISSN:1044-7431
1095-9327
DOI:10.1006/mcne.1994.1081