An anchored molecular map of mouse chromosome 6 with an analysis of interference

The relative simplicity of PCR-based microsatellite analysis has led to its being the method of choice for the definition of chromosomal regions associated with a mutation or disease. It has, therefore, become essential for the microsatellite map and the classical genetic map to be accurately integrated in order to facilitate the progression from genetic locus to candidate gene. One of the most effective ways to achieve this comprehensive molecular map of the mouse genome is to score microsatellites together with the classical molecular markers in the same cross. As a part of a genetic investigation of diabetes susceptibility in the NOD mouse, we have analyzed ten simple sequence repeats (SSR) markers on Chr 6 for correlation with the appearance of disease.