Molecular cloning and expression of a human heart inward rectifier potassium channel

WE have isolated a cDNA encoding an inwardly rectifying K+ channel (HH-IRK1) from human heart. The cDNA codes for a 427-amino acid protein, with two putative transmembrane domains and an H5 region. The primary structure of HH-IRK1 is homologous to that of the IRK1 channel cloned from a mouse macroph...

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Veröffentlicht in:	Neuroreport 1994-12, Vol.5 (18), p.2501-2505
Hauptverfasser:	Raab-Graham, Kimberly F, Radeke, Carolyn M, Vandenberg, Carol A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Cell membranes. Ionic channels. Membrane pores Cell structures and functions Chromosome Mapping Cloning, Molecular Electrophysiology Fundamental and applied biological sciences. Psychology Humans Molecular and cellular biology Molecular Sequence Data Myocardium - metabolism Oligonucleotide Probes - genetics Potassium Channels - genetics Potassium Channels - metabolism Potassium Channels - physiology RNA, Messenger - metabolism Tissue Distribution Xenopus
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Beschreibung
Zusammenfassung:	WE have isolated a cDNA encoding an inwardly rectifying K+ channel (HH-IRK1) from human heart. The cDNA codes for a 427-amino acid protein, with two putative transmembrane domains and an H5 region. The primary structure of HH-IRK1 is homologous to that of the IRK1 channel cloned from a mouse macrophage-like cell line. Functional expression in Xenopus oocytes showed that HH-IRK1 is a K+ channel with strong inward rectification, blocked by extracellular Ba and Cs, and with a single-channel conductance of 30 pS. Northern analysis showed HH-IRK1 transcripts in human heart, brain, skeletal muscle, placenta, lung and kidney. HH-IRK1 was mapped to human chromosome 17.
ISSN:	0959-4965 1473-558X
DOI:	10.1097/00001756-199412000-00024