A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)

Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibod...

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Veröffentlicht in:	BioSystems 1995, Vol.35 (2), p.183-188
Hauptverfasser:	Eray, Mete, Dogan, Numan S., Reiken, Steven R., Sutisna, Himawan, Van Wie, Bernard J., Koch, Alan R., Moffett, David F., Silber, Moris, Davis, William C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bispecific Antibodies, Monoclonal Bilayer lipid membranes Biosensing Techniques Biosensor Bispecific antibodies Drug Stability Ion Channels Lipid Bilayers Membrane Potentials Membranes, Artificial Microfabrication Modified nicotinic acetylcholine receptor Polymers Rats Receptors, Nicotinic - chemistry Receptors, Nicotinic - immunology Receptors, Nicotinic - isolation & purification Torpedo
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Beschreibung
Zusammenfassung:	Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.
ISSN:	0303-2647 1872-8324
DOI:	10.1016/0303-2647(94)01511-5