Phosphatidylcholine enhances the acrosomal responsiveness of human sperm

Supplementing bovine serum albumin‐containing medium with phosphatidylcholine (PC) accelerated the in vitro development of human sperm acrosomal responsiveness. Responsiveness was assessed by exposing the sperm to progesterone. The maximum effect was produced by incubation with 100 μ/ml, which resulted in 40% (23–56%) (mean, 95% confidence limits) of the sperm becoming responsive to progesterone at 24 hours, compared to 23% (10–40%) of control sperm. Enhancement was apparent after as little as 6 hours of incubation in vitro, and the number of responsive sperm was still increasing at the last time point tested (30 hours). PC had no apparent ill effects; it did not alter the percentage of motile sperm or the percentage of sperm stained with the supravital stain, Hoechst 33258. Enhanced responsiveness required prolonged incubation in PC, because PC was not effective when it was only applied at the same time as progesterone. Lysophosphatidyicholine did not enhance acrosomal responsiveness when used at concentrations from 10 ng/ml to 100 μg/ml, indicating that the effect of PC was not due to trace amounts of lysophosphatidylcholine. PC also increased the response of sperm to the Ca2+/H+‐exchanging ionophore, ionomycin, suggesting that PC modifies an event that is coincident with or subsequent to the rise in intracellular free Ca2+ that is triggered by progesterone.