Characterization and comparison of the polyclonal immune response to purified PLP and DM-20 in the lewis rat: Determination of a new antigenic determinant

Lewis rat polyclonal antibodies raised against pure PLP and DM-20 were characterized by indirect and competitive ELISA assays. Anti-PLP antibodies showed a good cross-reactivity with DM-20 and vice versa, suggesting that the chief B-cell epitopes are the same on both proteolipids. Three antigenic de...

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Veröffentlicht in:Neurochemistry international 1994-11, Vol.25 (5), p.433-440
Hauptverfasser: Klinguer, Christine, Trifilieff, Elisabeth
Format: Artikel
Sprache:eng
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Zusammenfassung:Lewis rat polyclonal antibodies raised against pure PLP and DM-20 were characterized by indirect and competitive ELISA assays. Anti-PLP antibodies showed a good cross-reactivity with DM-20 and vice versa, suggesting that the chief B-cell epitopes are the same on both proteolipids. Three antigenic determinants, PLP 57–70, PLP 200–218 and carboxyl-terminal sequence PLP 262–276, were identified for both proteolipids. Peptide PLP 57–70 represents a new B-cell epitope. The major hydrophilic loop (PLP 116–150), deleted in DM-20, did not show any antigenic activity. The study of polyclonal immune response to PLP and DM-20 will be helpful in resolving their potential role in the development and course of experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS).
ISSN:0197-0186
1872-9754
DOI:10.1016/0197-0186(94)90019-1