Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis

Capillary electrophoresis was used for the analysis of recombinant human tumor necrosis factor beta (rhTNF‐β). Reproducible separation of the protein could be achieved in Polybrene‐coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybr...

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Veröffentlicht in:	Electrophoresis 1995, Vol.16 (1), p.647-653
Hauptverfasser:	Yao, Yi Ju, Loh, Kean Chong, Chung, Maxey C. M., Li, Sam F. Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Capillary electrophoresis Electrophoresis - methods Hexadimethrine Bromide - chemistry Humans Lymphotoxin-alpha - analysis Recombinant Fusion Proteins - analysis Recombinant-DNA technology Tumor necrosis factor
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Zusammenfassung:	Capillary electrophoresis was used for the analysis of recombinant human tumor necrosis factor beta (rhTNF‐β). Reproducible separation of the protein could be achieved in Polybrene‐coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybrene‐coated capillaries, other commercially available column and column‐coating reagents were also examined for the separation of rhTNF‐β. Capillary electrophoresis was found to be a potentially useful method for product analysis and process monitoring in recombinant‐DNA technology.
ISSN:	0173-0835 1522-2683
DOI:	10.1002/elps.11501601104