Polyclonal Antiidiotypic Antibodies Mimicking gp120 of HIV-1

Rabbit antiidiotypic antibodies (Ab2) were produced against anti-HIV-1 antibody 0.5β (Ab1), which binds to gp120 of HIV-1 and shows virus-neutralizing activity. The Ab2 bound specifically to the Ab1 and their binding to Ab2 was inhibited by a recombinant fragment of gp120 (PB1) or a peptide (residue...

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Veröffentlicht in:Viral immunology 1994, Vol.7 (2), p.61-69
Hauptverfasser: SPERLAGH, M, HOXIE, J, MARUYAMA, H, STEFANO, K, GONZALES-SCARANO, F, PREWETT, M, SHAONONG LIANG, MATSUSHITA, S, HERLYN, D
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Sprache:eng
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Zusammenfassung:Rabbit antiidiotypic antibodies (Ab2) were produced against anti-HIV-1 antibody 0.5β (Ab1), which binds to gp120 of HIV-1 and shows virus-neutralizing activity. The Ab2 bound specifically to the Ab1 and their binding to Ab2 was inhibited by a recombinant fragment of gp120 (PB1) or a peptide (residues 301–324 of gp120), both expressing the Ab1-defined epitope. The Ab2 induced in rats antiantiidiotypic antibodies (Ab3) that were Ab1-like in their binding reactivities to PB1, native gp120 or peptide and shared idiotopes with the Ab1. However, the Ab2 did not induce virus-neutralizing Ab3, probably a reflection of the low avidity of the Ab3 as compared to the Ab1.
ISSN:0882-8245
1557-8976
DOI:10.1089/vim.1994.7.61