Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide
The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosacchar...
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| Veröffentlicht in: | Carbohydrate research 1995-11, Vol.277 (2), p.245-255 |
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| container_title | Carbohydrate research |
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| creator | Gamian, Andrzej Katzenellenbogen, Ewa Romanowska, Elzbieta García Fernández, JoséManuel Pedersen, Christian Ulrich, Jacques Defaye, Jacques |
| description | The lipopolysaccharide was extracted from cells of
Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide:
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| doi_str_mv | 10.1016/0008-6215(95)00200-D |
| format | Article |
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Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide:
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Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide:
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Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide:
▪</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>8556734</pmid><doi>10.1016/0008-6215(95)00200-D</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Carbohydrate research, 1995-11, Vol.277 (2), p.245-255 |
| issn | 0008-6215 1873-426X |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | antigens Carbohydrate Conformation Carbohydrate Sequence composition Endotoxin endotoxins Enterobacteriaceae - chemistry Hafnia alvei Lipopolysaccharide lipopolysaccharides LPS Magnetic Resonance Spectroscopy Mass Spectrometry methylation Molecular Sequence Data monosaccharides Monosaccharides - analysis O Antigens - chemistry O-Antigen Oligosaccharides - chemistry Repetitive Sequences, Nucleic Acid spectral analysis structure |
| title | Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide |
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