Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide

Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide

The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosacchar...

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Veröffentlicht in:Carbohydrate research 1995-11, Vol.277 (2), p.245-255
Hauptverfasser: Gamian, Andrzej, Katzenellenbogen, Ewa, Romanowska, Elzbieta, García Fernández, JoséManuel, Pedersen, Christian, Ulrich, Jacques, Defaye, Jacques
Format: Artikel
Sprache:eng
Schlagworte:
antigens
Carbohydrate Conformation
Carbohydrate Sequence
composition
Endotoxin
endotoxins
Enterobacteriaceae - chemistry
Hafnia alvei
Lipopolysaccharide
lipopolysaccharides
LPS
Magnetic Resonance Spectroscopy
Mass Spectrometry
methylation
Molecular Sequence Data
monosaccharides
Monosaccharides - analysis
O Antigens - chemistry
O-Antigen
Oligosaccharides - chemistry
Repetitive Sequences, Nucleic Acid
spectral analysis
structure
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Zusammenfassung:The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide: ▪
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(95)00200-D