Mono-, di- and trimeric binding of a bis-netropsin to DNA

An unusual 3:1 stoichiometry for complex formation between an elongated bis-netropsin compound and its binding site on DNA has been observed. Circular dichroism measurements distinguish two types of complexes formed between this bisnetropsin and poly[d(A-T)] · poly[d(A-T)]. The first type is charact...

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Veröffentlicht in:FEBS letters 1995-11, Vol.375 (3), p.304-306
Hauptverfasser: Zasedatelev, A.S., Borodulin, V.B., Grokhovsky, S.L., Nikitin, A.M., Salmanova, D.V., Zhuze, A.L., Gursky, G.V., Shafer, R.H.
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Sprache:eng
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Zusammenfassung:An unusual 3:1 stoichiometry for complex formation between an elongated bis-netropsin compound and its binding site on DNA has been observed. Circular dichroism measurements distinguish two types of complexes formed between this bisnetropsin and poly[d(A-T)] · poly[d(A-T)]. The first type is characterized by a 1:1 saturating ratio of bound molecules per ten base pairs. Formation of the second type results from the cooperative binding of two additional bis-netropsin molecules to the first type of complex. In contrast to these results observed for binding to the alternating polynucleotide, only the 1:1 type of complex is formed when this ligand binds to the homopolymer poly(dA) · poly(dT).
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01230-C