Isolation of a Human YAC Contig Encompassing a Cluster of UGT2 Genes and Its Regional Localization to Chromosome 4q13

Isolation of a Human YAC Contig Encompassing a Cluster of UGT2 Genes and Its Regional Localization to Chromosome 4q13

Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell bybrid cell lines. A ye...

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Veröffentlicht in:Genomics 1994-09, Vol.23 (2), p.496-499
Hauptverfasser: Monaghan, Gemma, Clarke, Douglas J., Povey, Sue, See, Chee Gee, Boxer, Maureen, Burchell, Brian
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Biological and medical sciences
BIOLOGY AND MEDICINE, BASIC STUDIES
Chromosome Mapping
Chromosomes, Artificial, Yeast
Chromosomes, Human, Pair 4
Classical genetics, quantitative genetics, hybrids
DNA HYBRIDIZATION
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
GENES
GENETIC MAPPING
Genetics of eukaryotes. Biological and molecular evolution
Glucuronosyltransferase - genetics
HEXOSYL TRANSFERASES
Human
HUMAN CHROMOSOMES
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Multigene Family
Polymerase Chain Reaction
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Zusammenfassung:Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell bybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15 . Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1994.1531