The use of capillary electrophoresis for the determination of hemoglobin variants

The application of capillary electrophoresis and related techniques for the detection of hemoglobin variants is described. Capillary zone electrophoresis (CZE) was applied for the analysis of intact tetrameric hemoglobin. CZE under denaturing conditions was used for the separation of globin chains....

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Veröffentlicht in:	Electrophoresis 1995, Vol.16 (1), p.1492-1498
Hauptverfasser:	Castagnola, Massimo, Messana, Irene, Cassiano, Loredana, Rabino, Rita, Rossetti, Diana V., Giardina, Bruno
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Capillary electrophoresis Capillary isoelectric focusing Capillary zone electrophoresis Chromatography - methods Chromatography, High Pressure Liquid Electrochemistry Electrophoresis, Capillary - methods Globins - isolation & purification Hemoglobin A - analysis Hemoglobin A - metabolism Hemoglobin C - analysis Hemoglobin C - metabolism Hemoglobin variants Hemoglobin, Sickle - analysis Hemoglobin, Sickle - metabolism Hemoglobins, Abnormal - analysis Hemoglobins, Abnormal - metabolism Humans Macromolecular Substances Micellar electrokinetic capillary chromatography Micelles Peptide Fragments - analysis Peptide Fragments - metabolism Peptide Mapping Trypsin - metabolism
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Zusammenfassung:	The application of capillary electrophoresis and related techniques for the detection of hemoglobin variants is described. Capillary zone electrophoresis (CZE) was applied for the analysis of intact tetrameric hemoglobin. CZE under denaturing conditions was used for the separation of globin chains. Both CZE and micellar electrokinetic capillary chromatography were applied for a fast and sensitive separation of tryptic digests and for the analysis of amino acid derivatives.
ISSN:	0173-0835 1522-2683
DOI:	10.1002/elps.11501601247