Development of a simple and selective separation of 67Cu from irradiated zinc for use in antibody labelling: A comparison of methods

Development of a simple and selective separation of 67Cu from irradiated zinc for use in antibody labelling: A comparison of methods

A procedure for the production and separation of Cu isotopes from irradiated Zn was developed. Following a comparison of methods based on extraction, electrolysis and ion-exchange chromatography, a technique for the separation of Cu employing three ion-exchange matrices was developed which was simpl...

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Veröffentlicht in:Applied radiation and isotopes 1995-05, Vol.46 (5), p.329-336
Hauptverfasser: Schwarzbach, Rolf, Zimmermann, Kurt, Bläuenstein, Peter, Smith, Alan, August Schubiger, P.
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies - chemistry
Chemistry Techniques, Analytical
Chromatography, Ion Exchange
Copper Radioisotopes - isolation & purification
Electrolysis
Evaluation Studies as Topic
Isotope Labeling - methods
Radiochemistry
Zinc - chemistry
Zinc - radiation effects
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Zusammenfassung:A procedure for the production and separation of Cu isotopes from irradiated Zn was developed. Following a comparison of methods based on extraction, electrolysis and ion-exchange chromatography, a technique for the separation of Cu employing three ion-exchange matrices was developed which was simple, reproducible and hot cell-compatible. The specific activity of the final product was 37 MBq 67Cu/μg Cu at EOB. The level of impurities was so low that no interference with antibody labelling was observed.
ISSN:0969-8043
1872-9800
DOI:10.1016/0969-8043(95)00010-B