Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments

A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO 2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and Sulfhemoglobin (SHb) in 35 μl blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm pe...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinica chimica acta 1987-07, Vol.166 (2), p.283-289
Hauptverfasser:	Fogh-Andersen, Niels, Siggaard-Andersen, Ole, Lundsgaard, Finn C., Wimberley, Peter D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Analytical, structural and metabolic biochemistry Biological and medical sciences Carboxyhemoglobin Carboxyhemoglobin - analysis Chromatography, Gas Fundamental and applied biological sciences. Psychology Gas chromatography Hemoglobins - analysis Hemoproteins Humans Metalloproteins Methemoglobin Methemoglobin - analysis Nephelometry and Turbidimetry Oxygen - blood Oxygen saturation Oxyhemoglobins - analysis Proteins Spectrophotometry - instrumentation Sulfhemoglobin Sulfhemoglobin - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO 2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and Sulfhemoglobin (SHb) in 35 μl blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm permitted the composition of any hemoglobin pigment mixture to be determined more accurately, precisely and easily than before. The inclusion of 670 nm, where the hemoglobin pigments have low absorption coefficients, allowed correction for turbidity.
ISSN:	0009-8981 1873-3492
DOI:	10.1016/0009-8981(87)90431-1