Primary structure of O- and N-glycosylic carbohydrate chains derived from murine submandibular mucin (MSM)

The carbohydrate moiety of mouse submandibular mucin (MSM) contains mainly d-mannose and 2-acetamido-2-deoxy- d-glucose together with sialic acid, d-galactose, and 2-acetamido-2-deoxy- d-galactose. O-Glycosylically bound saccharides, obtained by treatment of MSM with alkaline borohydride, were shown...

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Veröffentlicht in:	Carbohydrate research 1987-07, Vol.164, p.43-48
Hauptverfasser:	Nieuw Amerongen, Arie V., Oderkerk, Clasina H., Roukema, Pieter A., Wolf, Johannes H., Lisman, Jan J.W., Overdijk, Bernard, Fournet, Bernard, Montreuil, Jean, Van Halbeek, Herman, Mutsaers, Johanna H.G.M., Vliegenthart, Johannes F.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Carbohydrates Fundamental and applied biological sciences. Psychology Glycosides Indicators and Reagents Methylation Mice Mucins - isolation & purification Other biological molecules Submandibular Gland - analysis
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Zusammenfassung:	The carbohydrate moiety of mouse submandibular mucin (MSM) contains mainly d-mannose and 2-acetamido-2-deoxy- d-glucose together with sialic acid, d-galactose, and 2-acetamido-2-deoxy- d-galactose. O-Glycosylically bound saccharides, obtained by treatment of MSM with alkaline borohydride, were shown by methylation analysis to have the structure: α-NeuAc-(2→3)-β-Gal-(1→3)-GalNAc-ol. N-Glycosylically bound saccharides obtained from MSM by hydrazinolysis, and analysed by 500-MHz 1H-n.m.r. spectroscopy, were shown to have the following comprehensive structures. ▪
ISSN:	0008-6215 1873-426X
DOI:	10.1016/0008-6215(87)80117-9