A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae

A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae

We have constructed a convenient new vector, YEp-DE, for the construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. The vector contains the yeast LEU2 gene, the 2 μ origin of replication, and a region from pUC18 that includes the amp r gene, the Escheric...

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Veröffentlicht in:Plasmid 1987-03, Vol.17 (2), p.171-172
Hauptverfasser: Khan, Muhammed Ishaq, Ecker, David J., Butt, Tauseef, Gorman, Jessica A., Crooke, Stanley T.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
cloning vectors
Cloning, Molecular - methods
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
GENE
gene expression
gene libraries
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
Genetic Vectors
Methods. Procedures. Technologies
PLASMIDE
PLASMIDIOS
PLASMIDS
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
VECTEUR
VECTORES
VECTORS
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:We have constructed a convenient new vector, YEp-DE, for the construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. The vector contains the yeast LEU2 gene, the 2 μ origin of replication, and a region from pUC18 that includes the amp r gene, the Escherichia coli origin of replication (ori), and the LacZ gene with multiple cloning sites. Five sites (Sac1, Sma1, BamH1, Sal1, Sph1) in this region are unique. This vector has advantages over similar yeast- E. coli shuttle vectors: small size (7291 bp, entirely sequenced), convenient cloning sites, and lacZ selection for detecting recombinant plasmids.
ISSN:0147-619X
1095-9890
DOI:10.1016/0147-619X(87)90024-2