Application of flow cytometry to immunohematology

A method is described for applying flow cytometry to the analysis of populations of red blood cells that have been sensitized, in vivo or in vitro, with IgG antibody. Specific insttrument settings and details of the method are given with sufficient explanation for practical use in immunohematologic...

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Veröffentlicht in:	Journal of immunological methods 1987-07, Vol.101 (1), p.127-131
Hauptverfasser:	Nance, Sandra J., Garratty, George
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigen-Antibody Reactions Antigens, Surface - analysis Biological and medical sciences Erythrocytes - classification Erythrocytes - cytology Erythrocytes - immunology Flow cytometry Flow Cytometry - methods Fluorescein-5-isothiocyanate Fluoresceins - pharmacology Fundamental and applied biological sciences. Psychology Fundamental immunology Hematologic Diseases - immunology Humans Immunobiology Immunoglobulin A - metabolism Immunoglobulin G - metabolism Immunoglobulin M - metabolism Immunohematology Immunological reactions in vitro Lasers Methods Protein Binding Red blood cell testing Serologic method Thiocyanates - pharmacology
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Beschreibung
Zusammenfassung:	A method is described for applying flow cytometry to the analysis of populations of red blood cells that have been sensitized, in vivo or in vitro, with IgG antibody. Specific insttrument settings and details of the method are given with sufficient explanation for practical use in immunohematologic investigations.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(87)90225-0