Low molecular weight allergens of the pollen of parietaria officinalis

The allergenic composition of a low mol. wt fraction of the pollen extract of Parietaria officinalis (PO) was investigated. Fraction C, that was eluted after oxytocin (mol. wt 1040) when the pollen extract was gel filtered on Sephadex or on Biogel, was cross-reactive in the RAST with the major aller...

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Veröffentlicht in:	Molecular immunology 1987-03, Vol.24 (3), p.305-312
Hauptverfasser:	Ruffilli, A., Oreste, U., Santonastaso, V., Abusco, A.Scotto D', Sacerdoti, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergens - isolation & purification Antigens, allergens Biological and medical sciences Chromatography, Gel Fundamental and applied biological sciences. Psychology Fundamental immunology Histamine Release Humans Hypersensitivity, Immediate - immunology Immediate hypersensitivity. Allergy. Anaphylaxis, etc Immunobiology Leukocytes - metabolism Molecular Weight Parietaria officinalis Pollen - immunology Radioallergosorbent Test Skin Tests
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Beschreibung
Zusammenfassung:	The allergenic composition of a low mol. wt fraction of the pollen extract of Parietaria officinalis (PO) was investigated. Fraction C, that was eluted after oxytocin (mol. wt 1040) when the pollen extract was gel filtered on Sephadex or on Biogel, was cross-reactive in the RAST with the major allergen P015 and was capable of eliciting histamine release from leukocytes of sensitive donors. RAST inhibition (RAST I) analysis of the eluate of gel filtration on Sephadex G-10 revealed several peaks of IgE binding activity. Analysis of fine specificity of response of individual patients carried out by skin-prick tests and by RAST I, revealed individual patterns of reactivity, indicating that allergens contained in fraction C were minor allergens.
ISSN:	0161-5890 1872-9142
DOI:	10.1016/0161-5890(87)90149-0