Enzymatic activation of the Bacillus sphaericus mosquito larvicidal toxin

Bacillus sphaericus insecticidal toxin was activated by trypsin, α-chymotrypsin, and mosquito gut homogenates to a form which was cytotoxic to cultured mosquito cells. Gut extracts from highly resistant Aedes aegypti larvae were as effective in activating this toxin as extracts from the highly sensi...

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Veröffentlicht in:	Journal of invertebrate pathology 1987-07, Vol.50 (1), p.40-44
Hauptverfasser:	Davidson, Elizabeth W., Bieber, Allan L., Meyer, Marian, Shellabarger, Constance
Format:	Artikel
Sprache:	eng
Schlagworte:	AEDES Aedes - drug effects Aedes - enzymology AEDES AEGYPTI Animals BACILLUS Bacillus sphaericus BACTERIAL INSECTICIDES Bacterial Toxins - pharmacology Biological and medical sciences Cell Survival - drug effects CHYMOTRYPSIN CONTROL DE INSECTOS CULEX Culex - drug effects Culex - enzymology CULEX QUINQUEFASCIATUS Digestive System - enzymology ENZIMAS ENZYME ENZYMES Fundamental and applied biological sciences. Psychology HIDROLASAS HYDROLASE HYDROLASES INSECT CONTROL Insecta INSECTICIDAS BACTERIANOS INSECTICIDE BACTERIEN Invertebrates Larva - drug effects Larva - enzymology LUTTE ANTI-INSECTE mosquito Pathology Peptide Hydrolases - pharmacology TOXINAS TOXINE TOXINS TRIPSINA TRYPSIN TRYPSINE α-chymotrypsin
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Beschreibung
Zusammenfassung:	Bacillus sphaericus insecticidal toxin was activated by trypsin, α-chymotrypsin, and mosquito gut homogenates to a form which was cytotoxic to cultured mosquito cells. Gut extracts from highly resistant Aedes aegypti larvae were as effective in activating this toxin as extracts from the highly sensitive species Culex quinquefasciatus. Activation altered the apparent molecular weight of the toxin by ca. 2–4 kDa.
ISSN:	0022-2011 1096-0805
DOI:	10.1016/0022-2011(87)90143-1