The utilization of biotin-antibiotin interaction for the detection of antibody to hepatitis B surface antigen (anti-HBs)

A biotin-antibiotin solid-phase enzyme-linked immunoassay for the detection and quantitation of the antibody to hepatitis B surface antigen (anti-HBs) is described. The assay utilizes hepatitis B surface antigen as a solid-phase ‘capture’ reagent and a mixture of biotinylated HBsAg and antibiotin-co...

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Veröffentlicht in:	Journal of virological methods 1987-05, Vol.16 (1), p.45-54
Hauptverfasser:	Mushahwar, Isa K., Spiezia, Karen Stuckmann
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-HBs Antibiotin Avidin-HRP Biological and medical sciences Biotin - immunology Biotin/antibiotin assay Biotin/avidin assay Biotinylated HBsAg Blood Donors Cross Reactions Enzyme-Linked Immunosorbent Assay - standards Fundamental and applied biological sciences. Psychology Hepatitis B Antibodies - analysis Hepatitis B Surface Antigens - analysis Humans Microbiology Techniques used in virology Virology
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Beschreibung
Zusammenfassung:	A biotin-antibiotin solid-phase enzyme-linked immunoassay for the detection and quantitation of the antibody to hepatitis B surface antigen (anti-HBs) is described. The assay utilizes hepatitis B surface antigen as a solid-phase ‘capture’ reagent and a mixture of biotinylated HBsAg and antibiotin-conjugated horseradish peroxidase as a detector reagent. The assay was compared to a commercial enzyme immunoassay (AUSAB EIA) which used the biotin-avidin system for anti-HBs detection. The two assays were found to measure the same molecules and to correlate well regarding anti-HBs titers.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(87)90030-9