Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera
A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen....
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1987-04, Vol.14 (4), p.377-384 |
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| container_end_page | 384 |
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| container_issue | 4 |
| container_start_page | 377 |
| container_title | Veterinary immunology and immunopathology |
| container_volume | 14 |
| creator | Justewicz, D.M. Magar, R. Marsolais, G. Lecomte, J. |
| description | A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained : a coefficient of correlation (r) of 0.63 (P |
| doi_str_mv | 10.1016/0165-2427(87)90039-0 |
| format | Article |
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A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained : a coefficient of correlation (r) of 0.63 (P<0.01) between BVDV-ELISA and SN titer in BVDV-seroconverted animals and 0.71 for all sera, including that from BVDV vaccinated animals; a sensitivity of 92%, a specificity of 91%, a concordance of 92%; and a demonstration of a parallel increase in BVDV-ELISA and SN titers in animals with seroconversion. 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A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained : a coefficient of correlation (r) of 0.63 (P<0.01) between BVDV-ELISA and SN titer in BVDV-seroconverted animals and 0.71 for all sera, including that from BVDV vaccinated animals; a sensitivity of 92%, a specificity of 91%, a concordance of 92%; and a demonstration of a parallel increase in BVDV-ELISA and SN titers in animals with seroconversion. 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A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained : a coefficient of correlation (r) of 0.63 (P<0.01) between BVDV-ELISA and SN titer in BVDV-seroconverted animals and 0.71 for all sera, including that from BVDV vaccinated animals; a sensitivity of 92%, a specificity of 91%, a concordance of 92%; and a demonstration of a parallel increase in BVDV-ELISA and SN titers in animals with seroconversion. The BVDV-ELISA permits a more complete evaluation of the humoral immune response to BVDV.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>3037768</pmid><doi>10.1016/0165-2427(87)90039-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Veterinary immunology and immunopathology, 1987-04, Vol.14 (4), p.377-384 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies, Viral - analysis Antigens, Viral - immunology Bovine Virus Diarrhea-Mucosal Disease - immunology Cattle Cattle Diseases - immunology Cell Line Diarrhea Viruses, Bovine Viral - immunology Enzyme-Linked Immunosorbent Assay Immunoglobulin G - analysis Neutralization Tests Pestivirus - immunology |
| title | Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera |
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