Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera

A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen....

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Veröffentlicht in:	Veterinary immunology and immunopathology 1987-04, Vol.14 (4), p.377-384
Hauptverfasser:	Justewicz, D.M., Magar, R., Marsolais, G., Lecomte, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antigens, Viral - immunology Bovine Virus Diarrhea-Mucosal Disease - immunology Cattle Cattle Diseases - immunology Cell Line Diarrhea Viruses, Bovine Viral - immunology Enzyme-Linked Immunosorbent Assay Immunoglobulin G - analysis Neutralization Tests Pestivirus - immunology
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Zusammenfassung:	A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained : a coefficient of correlation (r) of 0.63 (P
ISSN:	0165-2427 1873-2534
DOI:	10.1016/0165-2427(87)90039-0