Interspersion of histone and 5S RNA genes in Artemia

Four recombinant λ phage containing histone genes were selected from a library of Artemia genomic DNA fragments. The histone gene organization of Artemia resembles that of other invertebrates in that all five genes are clustered and repeated in tandem with approximate repeat lengths of 8.5 kb and 9....

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Veröffentlicht in:	Gene 1987, Vol.51 (1), p.61-67
Hauptverfasser:	Andrews, Matthew T., Vaughn, Jack C., Perry, Brian A., Bagshaw, Joseph C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Artemia Artemia - genetics Artemia - growth & development Biological and medical sciences brine shrimp crustacean DNA hybridization DNA, Recombinant evolution Fundamental and applied biological sciences. Psychology gene families Genes Genes. Genome Histones - genetics Marine Molecular and cellular biology Molecular genetics Multigene Family phage λ vectors Recombinant DNA repeated sequences Repetitive Sequences, Nucleic Acid RNA, Ribosomal - genetics
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Zusammenfassung:	Four recombinant λ phage containing histone genes were selected from a library of Artemia genomic DNA fragments. The histone gene organization of Artemia resembles that of other invertebrates in that all five genes are clustered and repeated in tandem with approximate repeat lengths of 8.5 kb and 9.3 kb. Each recombinant λ phage isolate hybridizes with five histone mRNAs and unexpectedly also with 5S ribosomal RNA. Hybridization kinetics have shown the number of histone genes to be about 95–100 copies per haploid genome. An identical number of copies was determined for a hybridization probe containing the 5S gene but no histone genes. We have not found any evidence for a separate set of repeated 5S genes outside this histone + 5S block.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(87)90474-4