Stability, Specific Binding Activity, and Plasma Concentration in Mice of an Oligodeoxynucleotide Modified at 5'-Terminal with Poly (ethylene glycol)

Oligodeoxynucleotide (ODN) composed of 15 nucleotides was modified at 5'-terminal phosphate with hexylamine linker and chemically activated poly (ethylene glycol). This derivative showed improved characteristics in terms of enzymatic stability, binding activity, and in vivo retention in mouse....

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Veröffentlicht in:	Biological & pharmaceutical bulletin 1995/03/15, Vol.18(3), pp.474-476
Hauptverfasser:	KAWAGUCHI, Takeo, ASAKAWA, Hiroki, TASHIRO, Yasutaka, JUNI, Kazuhiko, SUEISHI, Toshihiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibiotics. Antiinfectious agents. Antiparasitic agents antisense Antiviral agents Base Sequence Biological and medical sciences Drug Stability Humans in vivo Injections, Intravenous Male Medical sciences Mice Mice, Inbred Strains modification Molecular Sequence Data oligodeoxynucleotide Oligonucleotides - blood Oligonucleotides - metabolism Oligonucleotides, Antisense - metabolism Pharmacology. Drug treatments poly (ethylene glycol) Polyethylene Glycols - pharmacology Sensitivity and Specificity Single-Strand Specific DNA and RNA Endonucleases - metabolism stability
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Beschreibung
Zusammenfassung:	Oligodeoxynucleotide (ODN) composed of 15 nucleotides was modified at 5'-terminal phosphate with hexylamine linker and chemically activated poly (ethylene glycol). This derivative showed improved characteristics in terms of enzymatic stability, binding activity, and in vivo retention in mouse. The data are discussed in comparison with those of corresponding unmodified and phosphorothioate ODNs.
ISSN:	0918-6158 1347-5215
DOI:	10.1248/bpb.18.474