Detection of rDNA sites in sugarcane by FISH

Hybridization sites of an rDNA probe coding for the 5.8S, 18S and 26S genes were detected on the chromosomes of sugarcane and a related genus, Erianthus, using fluorescence in situ hybridization. One unpaired and five paired hybridization sites were detected in a Saccharum spp. hybrid. A first intro...

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Veröffentlicht in:Chromosome research 1995-11, Vol.3 (7), p.444-445
Hauptverfasser: Jenkin, M.J, Reader, S.M, Purdie, K.A, Miller, T.E
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Reader, S.M
Purdie, K.A
Miller, T.E
description Hybridization sites of an rDNA probe coding for the 5.8S, 18S and 26S genes were detected on the chromosomes of sugarcane and a related genus, Erianthus, using fluorescence in situ hybridization. One unpaired and five paired hybridization sites were detected in a Saccharum spp. hybrid. A first introgression hybrid (I1) between Saccharum officinarum and Saccharum spontaneum had seven pairs of hybridization sites. A clone of Erianthus arundinaceus showed six hybridization sites in somatic tissue.
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subjects Australia
chromosomes
Chromosomes - genetics
DNA Probes
DNA, Ribosomal - genetics
erianthus arundinaceus
fluorescence in situ hybridization
Fluorescent Dyes
genes
hybrids
In Situ Hybridization, Fluorescence
Indoles
interspecific hybridization
Meiosis
Metaphase
nucleic acid hybridization
nucleolus organizer
Nucleolus Organizer Region - genetics
Plants - genetics
ribosomal DNA
ribosomal RNA
Saccharum
Saccharum officinarum
Saccharum spontaneum
title Detection of rDNA sites in sugarcane by FISH
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