Detection of rDNA sites in sugarcane by FISH

Hybridization sites of an rDNA probe coding for the 5.8S, 18S and 26S genes were detected on the chromosomes of sugarcane and a related genus, Erianthus, using fluorescence in situ hybridization. One unpaired and five paired hybridization sites were detected in a Saccharum spp. hybrid. A first intro...

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Veröffentlicht in:	Chromosome research 1995-11, Vol.3 (7), p.444-445
Hauptverfasser:	Jenkin, M.J, Reader, S.M, Purdie, K.A, Miller, T.E
Format:	Artikel
Sprache:	eng
Schlagworte:	Australia chromosomes Chromosomes - genetics DNA Probes DNA, Ribosomal - genetics erianthus arundinaceus fluorescence in situ hybridization Fluorescent Dyes genes hybrids In Situ Hybridization, Fluorescence Indoles interspecific hybridization Meiosis Metaphase nucleic acid hybridization nucleolus organizer Nucleolus Organizer Region - genetics Plants - genetics ribosomal DNA ribosomal RNA Saccharum Saccharum officinarum Saccharum spontaneum
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Beschreibung
Zusammenfassung:	Hybridization sites of an rDNA probe coding for the 5.8S, 18S and 26S genes were detected on the chromosomes of sugarcane and a related genus, Erianthus, using fluorescence in situ hybridization. One unpaired and five paired hybridization sites were detected in a Saccharum spp. hybrid. A first introgression hybrid (I1) between Saccharum officinarum and Saccharum spontaneum had seven pairs of hybridization sites. A clone of Erianthus arundinaceus showed six hybridization sites in somatic tissue.
ISSN:	0967-3849 1573-6849
DOI:	10.1007/bf00713896