Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [ 3H]dihydrorotenone

Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [ 3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of M r 33 000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was estab...

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Veröffentlicht in:	FEBS letters 1987-07, Vol.219 (1), p.108-112
Hauptverfasser:	Earley, Fergus G.P., Patel, Salil D., Ragan, C.Ian, Attardi, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Cytochrome Reductases - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Immunochemistry Immunologic Techniques Mitochondria, Heart - enzymology Mitochondrial genome NADH dehydrogenase NADH Dehydrogenase - antagonists & inhibitors NADH Dehydrogenase - genetics NADH Dehydrogenase - metabolism ND-1 gene product Oxidoreductases Photochemistry Protein Binding Rotenone Rotenone - analogs & derivatives Rotenone - metabolism
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Zusammenfassung:	Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [ 3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of M r 33 000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(87)81200-0