In vivo release of interleukin-1β into hypothalamic extracellular fluid in rats: effects of repeated sampling
Interleukin-1β (Il-1β) concentrations in extracellular fluid (ECF) withdrawn at 10-min intervals through a push-pull cannula (PPC) located in the hypothalamus were studied in freely behaving male rats for 1 h at 24 and 72 h and again at 7 days after PPC implantation. Il-1β concentrations in ECF were...
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Veröffentlicht in: | Brain research 1995-08, Vol.688 (1), p.193-197 |
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Sprache: | eng |
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Zusammenfassung: | Interleukin-1β (Il-1β) concentrations in extracellular fluid (ECF) withdrawn at 10-min intervals through a push-pull cannula (PPC) located in the hypothalamus were studied in freely behaving male rats for 1 h at 24 and 72 h and again at 7 days after PPC implantation. Il-1β concentrations in ECF were similar in the latter. However, when ECF was sampled at 3 h and again 7 days after PPC implantation, Il-1β concentrations were greatly elevated at 7 days when compared to all other intervals. These results demonstrate how the relationships between Il-1β measured in ECF and the conditions of measurement appear to be integral parts of a whole intracerebral system: cytokine concentrations appear to be inextricably bound to intrahypothalamic conditions created by the sampling device presence and frequency of use. |
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ISSN: | 0006-8993 1872-6240 |
DOI: | 10.1016/0006-8993(95)00491-8 |