In vivo release of interleukin-1β into hypothalamic extracellular fluid in rats: effects of repeated sampling

Interleukin-1β (Il-1β) concentrations in extracellular fluid (ECF) withdrawn at 10-min intervals through a push-pull cannula (PPC) located in the hypothalamus were studied in freely behaving male rats for 1 h at 24 and 72 h and again at 7 days after PPC implantation. Il-1β concentrations in ECF were...

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Veröffentlicht in:Brain research 1995-08, Vol.688 (1), p.193-197
Hauptverfasser: Phelps, C.P., Sahu, A., Oliver, J., Ma, X.-L., Kalra, P.S., Kalra, S.P., Menzies, R.A., Chen, L., Denker, M., Horvath, E., Poole, L.L.
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Sprache:eng
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Zusammenfassung:Interleukin-1β (Il-1β) concentrations in extracellular fluid (ECF) withdrawn at 10-min intervals through a push-pull cannula (PPC) located in the hypothalamus were studied in freely behaving male rats for 1 h at 24 and 72 h and again at 7 days after PPC implantation. Il-1β concentrations in ECF were similar in the latter. However, when ECF was sampled at 3 h and again 7 days after PPC implantation, Il-1β concentrations were greatly elevated at 7 days when compared to all other intervals. These results demonstrate how the relationships between Il-1β measured in ECF and the conditions of measurement appear to be integral parts of a whole intracerebral system: cytokine concentrations appear to be inextricably bound to intrahypothalamic conditions created by the sampling device presence and frequency of use.
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)00491-8