A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells
The growth hormone (GH) gene is specifically expressed in the anterior pituitary. The first 289 bp of the human (h)GH 5′ region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH...
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| Veröffentlicht in: | Cell 1987-07, Vol.50 (2), p.267-275 |
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| creator | Bodner, Mordechai Karin, Michael |
| description | The growth hormone (GH) gene is specifically expressed in the anterior pituitary. The first 289 bp of the human (h)GH 5′ region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH TATA box. Using in vitro transcription systems we demonstrate greater hGH promoter activity in extracts of GH-expressing GC cells than in extracts of nonexpressing HeLa cells. The two GHF-1 binding sites are essential promoter elements in GC extracts. Addition of GHF-1-containing fractions to HeLa extracts stimulates hGH promoter activity. Thus, GHF-1 appears to be a positively acting, cell-type- and promoter-specific transcription factor. Furthermore, the tissue specificity of the hGH promoter appears to be positively controlled by the abundance or activity of GHF-1. |
| doi_str_mv | 10.1016/0092-8674(87)90222-4 |
| format | Article |
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The first 289 bp of the human (h)GH 5′ region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH TATA box. Using in vitro transcription systems we demonstrate greater hGH promoter activity in extracts of GH-expressing GC cells than in extracts of nonexpressing HeLa cells. The two GHF-1 binding sites are essential promoter elements in GC extracts. Addition of GHF-1-containing fractions to HeLa extracts stimulates hGH promoter activity. Thus, GHF-1 appears to be a positively acting, cell-type- and promoter-specific transcription factor. Furthermore, the tissue specificity of the hGH promoter appears to be positively controlled by the abundance or activity of GHF-1.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(87)90222-4</identifier><identifier>PMID: 3594572</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Fundamental and applied biological sciences. Psychology ; Genes ; Growth Hormone - genetics ; HeLa Cells - drug effects ; Humans ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Pituitary Gland, Anterior - metabolism ; Pituitary Neoplasms ; Promoter Regions, Genetic ; Rats ; Templates, Genetic ; Transcription Factors - physiology ; Transcription, Genetic - drug effects ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Cell, 1987-07, Vol.50 (2), p.267-275</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-5ed07951bcbcedcd7e70bee795e11044c8ce9d29ffdcfcd9af4712548aa723ba3</citedby><cites>FETCH-LOGICAL-c483t-5ed07951bcbcedcd7e70bee795e11044c8ce9d29ffdcfcd9af4712548aa723ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(87)90222-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7467564$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3594572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bodner, Mordechai</creatorcontrib><creatorcontrib>Karin, Michael</creatorcontrib><title>A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells</title><title>Cell</title><addtitle>Cell</addtitle><description>The growth hormone (GH) gene is specifically expressed in the anterior pituitary. The first 289 bp of the human (h)GH 5′ region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH TATA box. Using in vitro transcription systems we demonstrate greater hGH promoter activity in extracts of GH-expressing GC cells than in extracts of nonexpressing HeLa cells. The two GHF-1 binding sites are essential promoter elements in GC extracts. Addition of GHF-1-containing fractions to HeLa extracts stimulates hGH promoter activity. Thus, GHF-1 appears to be a positively acting, cell-type- and promoter-specific transcription factor. Furthermore, the tissue specificity of the hGH promoter appears to be positively controlled by the abundance or activity of GHF-1.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Growth Hormone - genetics</subject><subject>HeLa Cells - drug effects</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>Pituitary Neoplasms</subject><subject>Promoter Regions, Genetic</subject><subject>Rats</subject><subject>Templates, Genetic</subject><subject>Transcription Factors - physiology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Genes</topic><topic>Growth Hormone - genetics</topic><topic>HeLa Cells - drug effects</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>Pituitary Neoplasms</topic><topic>Promoter Regions, Genetic</topic><topic>Rats</topic><topic>Templates, Genetic</topic><topic>Transcription Factors - physiology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodner, Mordechai</creatorcontrib><creatorcontrib>Karin, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodner, Mordechai</au><au>Karin, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1987-07-17</date><risdate>1987</risdate><volume>50</volume><issue>2</issue><spage>267</spage><epage>275</epage><pages>267-275</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>The growth hormone (GH) gene is specifically expressed in the anterior pituitary. The first 289 bp of the human (h)GH 5′ region contains a promoter that is active only in pituitary-derived cell lines. These cell lines also contain a specific factor, GHF-1, that binds to two sites upstream to the hGH TATA box. Using in vitro transcription systems we demonstrate greater hGH promoter activity in extracts of GH-expressing GC cells than in extracts of nonexpressing HeLa cells. The two GHF-1 binding sites are essential promoter elements in GC extracts. Addition of GHF-1-containing fractions to HeLa extracts stimulates hGH promoter activity. Thus, GHF-1 appears to be a positively acting, cell-type- and promoter-specific transcription factor. Furthermore, the tissue specificity of the hGH promoter appears to be positively controlled by the abundance or activity of GHF-1.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>3594572</pmid><doi>10.1016/0092-8674(87)90222-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | Cell, 1987-07, Vol.50 (2), p.267-275 |
| issn | 0092-8674 1097-4172 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cell Line Fundamental and applied biological sciences. Psychology Genes Growth Hormone - genetics HeLa Cells - drug effects Humans Molecular and cellular biology Molecular genetics Mutation Pituitary Gland, Anterior - metabolism Pituitary Neoplasms Promoter Regions, Genetic Rats Templates, Genetic Transcription Factors - physiology Transcription, Genetic - drug effects Transcription. Transcription factor. Splicing. Rna processing |
| title | A pituitary-specific trans-acting factor can stimulate transcription from the growth hormone promoter in extracts of nonexpressing cells |
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