Rolling in P-Selectin–Deficient Mice Is Reduced But Not Eliminated in the Dorsal Skin

P-selectin–mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SO vessels of P-selectin–deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin–deficient mice was significantly lower than that in wild-type mice under the basal conditions and post–ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin–deficient and wild-type mice in shear rate or leukocyte-endo-thelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin–mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.