Inhibition of nitric oxide (NO •) production in murine macrophages by flavones
The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production of nitrite by murine activated peritoneal macrophages was studied in vitro. Activated peritoneal macrophages obtained from mice pre-treated with concanavalin A (Con A) ( in vivo), after exposure i...
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Veröffentlicht in: | Biochemical pharmacology 1995-09, Vol.50 (7), p.1031-1035 |
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Sprache: | eng |
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Zusammenfassung: | The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production of nitrite by murine activated peritoneal macrophages was studied
in vitro. Activated peritoneal macrophages obtained from mice pre-treated with concanavalin A (Con A) (
in vivo), after exposure
in vitro to lipopolysaccharide (LPS) at a concentration of 100 ng/ml, produced nitrite (20.3 ± 2.5 nmol/10
6 cells), as measured after 24 hr by the Griess reaction. Stimulation of production of nitrite was inhibited by N
G-mono-methyl-L-arginine, suggesting that nitrite was formed via nitric oxide (NO
•) as a product of metabolism of arginine. Stimulation was inhibited by flavone and the aminoflavones (20–100 μM). 3′-amino-4′-hydroxyflavone was the most potent inhibitor of nitrite production. Genistein (5,7-dihydroxy-3-(4-hydroxy-phenyl)-4H-1-ben-zopyran-4-one) also inhibited production of nitrite, by a mechanism that appears not to involve protein tyrosine kinases. These results suggest that the flavones can modulate the immune responses and the inflammatory reactions by controlling production of nitric oxide. |
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ISSN: | 0006-2952 1873-2968 |
DOI: | 10.1016/0006-2952(95)00237-T |