Inhibition of nitric oxide (NO •) production in murine macrophages by flavones

The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production of nitrite by murine activated peritoneal macrophages was studied in vitro. Activated peritoneal macrophages obtained from mice pre-treated with concanavalin A (Con A) ( in vivo), after exposure i...

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Veröffentlicht in:Biochemical pharmacology 1995-09, Vol.50 (7), p.1031-1035
Hauptverfasser: Król, Wojciech, Czuba, Zenon P., Threadgill, Michael D., Cunningham, Bernadette D.M., Pietsz, Grazyna
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Sprache:eng
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Zusammenfassung:The effect of flavone (2-phenylbenzopyran-4-one) and three amino-substituted flavones on the production of nitrite by murine activated peritoneal macrophages was studied in vitro. Activated peritoneal macrophages obtained from mice pre-treated with concanavalin A (Con A) ( in vivo), after exposure in vitro to lipopolysaccharide (LPS) at a concentration of 100 ng/ml, produced nitrite (20.3 ± 2.5 nmol/10 6 cells), as measured after 24 hr by the Griess reaction. Stimulation of production of nitrite was inhibited by N G-mono-methyl-L-arginine, suggesting that nitrite was formed via nitric oxide (NO •) as a product of metabolism of arginine. Stimulation was inhibited by flavone and the aminoflavones (20–100 μM). 3′-amino-4′-hydroxyflavone was the most potent inhibitor of nitrite production. Genistein (5,7-dihydroxy-3-(4-hydroxy-phenyl)-4H-1-ben-zopyran-4-one) also inhibited production of nitrite, by a mechanism that appears not to involve protein tyrosine kinases. These results suggest that the flavones can modulate the immune responses and the inflammatory reactions by controlling production of nitric oxide.
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(95)00237-T