Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary
In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/- 0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations...
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| Veröffentlicht in: | Biology of reproduction 1987-04, Vol.36 (3), p.536-542 |
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| container_title | Biology of reproduction |
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| creator | J H Sogn T E Curry, Jr M Brännström W J Lemaire R D Koos H Papkoff P O Janson |
| description | In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine
follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/-
0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations per ovary, respectively). Indomethacin (5 micrograms/ml) added to the perfusate
inhibited this ovulatory effect and exogenous prostaglandin F2 alpha (PGF2 alpha) (1 microgram/ml), or prostaglandin E2 (PGE2)
(0.5 microgram/ml), reversed the blockade. Ovine FSH and rFSH had only a weak stimulatory effect on estradiol release, and
only rFSH caused a significant increase in progesterone accumulation. Indomethacin reduced the stimulatory effect of rFSH
on progesterone release, and this effect was reversed by PGE2 but not by PGF2 alpha. In a 6-h incubation experiment with preovulatory
rat follicles, we tested the biological activity of gonadotropins used to induce oocyte maturation. The concentration of FSH
used in the perfusion experiments induced oocyte maturation in more than 88% of the oocytes studied. The data confirm earlier
findings that FSH can induce ovulations and show that prostaglandins are involved in this process. The data also indicate
that prostaglandins might be involved in the FSH-induced increase of progesterone levels. |
| doi_str_mv | 10.1095/biolreprod36.3.536 |
| format | Article |
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follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/-
0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations per ovary, respectively). Indomethacin (5 micrograms/ml) added to the perfusate
inhibited this ovulatory effect and exogenous prostaglandin F2 alpha (PGF2 alpha) (1 microgram/ml), or prostaglandin E2 (PGE2)
(0.5 microgram/ml), reversed the blockade. Ovine FSH and rFSH had only a weak stimulatory effect on estradiol release, and
only rFSH caused a significant increase in progesterone accumulation. Indomethacin reduced the stimulatory effect of rFSH
on progesterone release, and this effect was reversed by PGE2 but not by PGF2 alpha. In a 6-h incubation experiment with preovulatory
rat follicles, we tested the biological activity of gonadotropins used to induce oocyte maturation. The concentration of FSH
used in the perfusion experiments induced oocyte maturation in more than 88% of the oocytes studied. The data confirm earlier
findings that FSH can induce ovulations and show that prostaglandins are involved in this process. The data also indicate
that prostaglandins might be involved in the FSH-induced increase of progesterone levels.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod36.3.536</identifier><identifier>PMID: 3109504</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Female ; Follicle Stimulating Hormone - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; In Vitro Techniques ; Indomethacin - pharmacology ; Mammalian female genital system ; Oogenesis - drug effects ; Ovary - drug effects ; Ovary - physiology ; Ovulation - drug effects ; Perfusion ; Progesterone - biosynthesis ; Prostaglandins - physiology ; Rats ; Rats, Inbred Strains ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1987-04, Vol.36 (3), p.536-542</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7461720$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3109504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>J H Sogn</creatorcontrib><creatorcontrib>T E Curry, Jr</creatorcontrib><creatorcontrib>M Brännström</creatorcontrib><creatorcontrib>W J Lemaire</creatorcontrib><creatorcontrib>R D Koos</creatorcontrib><creatorcontrib>H Papkoff</creatorcontrib><creatorcontrib>P O Janson</creatorcontrib><title>Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine
follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/-
0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations per ovary, respectively). Indomethacin (5 micrograms/ml) added to the perfusate
inhibited this ovulatory effect and exogenous prostaglandin F2 alpha (PGF2 alpha) (1 microgram/ml), or prostaglandin E2 (PGE2)
(0.5 microgram/ml), reversed the blockade. Ovine FSH and rFSH had only a weak stimulatory effect on estradiol release, and
only rFSH caused a significant increase in progesterone accumulation. Indomethacin reduced the stimulatory effect of rFSH
on progesterone release, and this effect was reversed by PGE2 but not by PGF2 alpha. In a 6-h incubation experiment with preovulatory
rat follicles, we tested the biological activity of gonadotropins used to induce oocyte maturation. The concentration of FSH
used in the perfusion experiments induced oocyte maturation in more than 88% of the oocytes studied. The data confirm earlier
findings that FSH can induce ovulations and show that prostaglandins are involved in this process. The data also indicate
that prostaglandins might be involved in the FSH-induced increase of progesterone levels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>In Vitro Techniques</subject><subject>Indomethacin - pharmacology</subject><subject>Mammalian female genital system</subject><subject>Oogenesis - drug effects</subject><subject>Ovary - drug effects</subject><subject>Ovary - physiology</subject><subject>Ovulation - drug effects</subject><subject>Perfusion</subject><subject>Progesterone - biosynthesis</subject><subject>Prostaglandins - physiology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU9PwzAMxSMEGmPwBZCQegBuHUndJOsRTfyZNIkLnKu0ddagtBlJy7RvT2ATJ8t-v2dZz4RcMzpntOAPlXHW49a7BsQc5hzECZkynhWpzMTilEwppSIFEHBOLkL4pJTlkMGETODXT_Mp0au-NZUZjOsTpxPtrDW1xTQMphutGky_SVrnO9djavpmrLFJ3PefEh3VPolD1-HQqtr0sUmGFpMtej2GSHo1RFr5_SU508oGvDrWGfl4fnpfvqbrt5fV8nGdtpngQ8oYyoJLlgnImyqnhQKFUmFBJeZIqRSc1xkDLYBjoRlghfkCF4rTQjd5ATNyf9gbQ_kaMQxlZ0KN1qoe3RhKKbkQCw4RvDmCY9VhU2696eKd5TGYqN8edRVqZbVXfW3CPyZzwWRGI3Z3wFqzaXfGYxk6ZW1cCuVutwNRQhnfAj-WHoJx</recordid><startdate>19870401</startdate><enddate>19870401</enddate><creator>J H Sogn</creator><creator>T E Curry, Jr</creator><creator>M Brännström</creator><creator>W J Lemaire</creator><creator>R D Koos</creator><creator>H Papkoff</creator><creator>P O Janson</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19870401</creationdate><title>Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary</title><author>J H Sogn ; T E Curry, Jr ; M Brännström ; W J Lemaire ; R D Koos ; H Papkoff ; P O Janson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-11e795712634db409a3ae7ae907e4e007655c213f635e9f13ebe48e8a509fd493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>In Vitro Techniques</topic><topic>Indomethacin - pharmacology</topic><topic>Mammalian female genital system</topic><topic>Oogenesis - drug effects</topic><topic>Ovary - drug effects</topic><topic>Ovary - physiology</topic><topic>Ovulation - drug effects</topic><topic>Perfusion</topic><topic>Progesterone - biosynthesis</topic><topic>Prostaglandins - physiology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>J H Sogn</creatorcontrib><creatorcontrib>T E Curry, Jr</creatorcontrib><creatorcontrib>M Brännström</creatorcontrib><creatorcontrib>W J Lemaire</creatorcontrib><creatorcontrib>R D Koos</creatorcontrib><creatorcontrib>H Papkoff</creatorcontrib><creatorcontrib>P O Janson</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>J H Sogn</au><au>T E Curry, Jr</au><au>M Brännström</au><au>W J Lemaire</au><au>R D Koos</au><au>H Papkoff</au><au>P O Janson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1987-04-01</date><risdate>1987</risdate><volume>36</volume><issue>3</issue><spage>536</spage><epage>542</epage><pages>536-542</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine
follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/-
0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations per ovary, respectively). Indomethacin (5 micrograms/ml) added to the perfusate
inhibited this ovulatory effect and exogenous prostaglandin F2 alpha (PGF2 alpha) (1 microgram/ml), or prostaglandin E2 (PGE2)
(0.5 microgram/ml), reversed the blockade. Ovine FSH and rFSH had only a weak stimulatory effect on estradiol release, and
only rFSH caused a significant increase in progesterone accumulation. Indomethacin reduced the stimulatory effect of rFSH
on progesterone release, and this effect was reversed by PGE2 but not by PGF2 alpha. In a 6-h incubation experiment with preovulatory
rat follicles, we tested the biological activity of gonadotropins used to induce oocyte maturation. The concentration of FSH
used in the perfusion experiments induced oocyte maturation in more than 88% of the oocytes studied. The data confirm earlier
findings that FSH can induce ovulations and show that prostaglandins are involved in this process. The data also indicate
that prostaglandins might be involved in the FSH-induced increase of progesterone levels.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>3109504</pmid><doi>10.1095/biolreprod36.3.536</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1987-04, Vol.36 (3), p.536-542 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77566853 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Female Follicle Stimulating Hormone - pharmacology Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation In Vitro Techniques Indomethacin - pharmacology Mammalian female genital system Oogenesis - drug effects Ovary - drug effects Ovary - physiology Ovulation - drug effects Perfusion Progesterone - biosynthesis Prostaglandins - physiology Rats Rats, Inbred Strains Vertebrates: reproduction |
| title | Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary |
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