Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary
In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/- 0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations...
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Veröffentlicht in: | Biology of reproduction 1987-04, Vol.36 (3), p.536-542 |
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Zusammenfassung: | In isolated, perfused ovaries of rats treated with pregnant mare's serum gonadotropin (PMSG), purified preparations of ovine
follicle-stimulating hormone (FSH) (oFSH-211B) and rat FSH (rFSH-I-6), 100 ng/ml, were found to induce ovulations (4.8 +/-
0.9, n = 4, and 6.4 +/- 2.0, n = 5, ovulations per ovary, respectively). Indomethacin (5 micrograms/ml) added to the perfusate
inhibited this ovulatory effect and exogenous prostaglandin F2 alpha (PGF2 alpha) (1 microgram/ml), or prostaglandin E2 (PGE2)
(0.5 microgram/ml), reversed the blockade. Ovine FSH and rFSH had only a weak stimulatory effect on estradiol release, and
only rFSH caused a significant increase in progesterone accumulation. Indomethacin reduced the stimulatory effect of rFSH
on progesterone release, and this effect was reversed by PGE2 but not by PGF2 alpha. In a 6-h incubation experiment with preovulatory
rat follicles, we tested the biological activity of gonadotropins used to induce oocyte maturation. The concentration of FSH
used in the perfusion experiments induced oocyte maturation in more than 88% of the oocytes studied. The data confirm earlier
findings that FSH can induce ovulations and show that prostaglandins are involved in this process. The data also indicate
that prostaglandins might be involved in the FSH-induced increase of progesterone levels. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod36.3.536 |