Localization of neuron-specific enolase (NSE) mRNA in human brain

Localization of neuron-specific enolase (NSE) mRNA in human brain

In situ hybridization with biotinylated DNA and RNA probes derived from the 3′ untranslated region of human neuron-specific or γγ-enolase (NSE) permits histochemical detection of NSE mRNA in autopsy samples of human brain. In both aged controls and Alzheimer's disease (AD) patients, NSE probe h...

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Veröffentlicht in:Neuroscience letters 1987-05, Vol.76 (2), p.233-238
Hauptverfasser: Schmechel, D.E., Marangos, P.J., Martin, B.M., Winfield, S., Burkhart, D.S., Roses, A.D., Ginns, E.I.
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Alzheimer's disease (AD)
Biological and medical sciences
Brain - metabolism
Cerebellum - metabolism
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
DNA
Human brain
Humans
Immunochemistry
In situ hybridization histochemistry
Isoenzymes - genetics
Liver - metabolism
Medical sciences
Neurology
Neuron-specific enolase (NSE)
Non-neuronal enolase (NNE)
Nucleic Acid Hybridization
Phosphopyruvate Hydratase - genetics
Rapid autopsy
RNA, Messenger - metabolism
Visual Cortex - metabolism
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Zusammenfassung:In situ hybridization with biotinylated DNA and RNA probes derived from the 3′ untranslated region of human neuron-specific or γγ-enolase (NSE) permits histochemical detection of NSE mRNA in autopsy samples of human brain. In both aged controls and Alzheimer's disease (AD) patients, NSE probe hybridized with variable intensity to nerve cells in visual cortex and cerebellum, but not to glial or liver cells. These results provide evidence that (1) NSE mRNA is expressed in neurons in human brain tissue, (2) pattern of NSE mRNA expression in unaffected brain regions in AD is qualitatively normal, and (3) NSE mRNA expression may vary widely among different classes of neurons.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(87)90721-X